Not known Facts About attune flow cytometer
Not known Facts About attune flow cytometer
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At that time, the early signals were dismissed as particles, nevertheless the retrospective evaluation demonstrated shared characteristics Along with the problematic cells in tradition. Tracing the an infection helped the lab set up further laboratory treatments for screening and security of assay-vital cell traces.
As samples are obtained within the Attune CytPix Flow Cytometer, the superior-speed brightfield digital camera captures and retailers photographs of detected activities in a charge nearly six,000 photographs for each next, with regards to the flow level and image dimensions. For higher adaptability, Attune Cytometric Software helps you to modify the graphic seize frequency as wanted and select a selected gate for image capture, if expected.
Blend acoustic concentrating know-how with superior-parameter Examination for versatile spectral unmixing and standard workflows.
Learn new insights into your experimental details by visualizing populations employing blended fluorescence and morphology info. Our application interface wizards permit downsampling, concatenation (merged data files) and dimensionality resources that happen to be created to the computer software for an intuitive, streamlined information Examination working experience.
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Listed here, a highly trained consumer has gated singlets confidently. Soon after analyzing the manual singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate is made up of much more than four% aggregates.
Pair your Attune flow cytometer using an autosampler for elevated workflow performance, and include robotic integration for max throughput.
Our scientists will design and style panels in your case as often times you may need—cost-free, no merchandise buy needed.
Early indications of this contamination experienced originally been dismissed as particles. Backgating to find out new populations. Backgating imaged cells on the Attune CytPix Flow Cytometer also helps you to use morphological characteristics to discover fascinating subpopulations that would not be obvious from flow cytometry info by yourself.
Possibly most importantly, these occasions comprise cells of Evidently distinct phenotypes which could lead on to incorrect conclusions pertaining to double good gatherings (specifically in rare populations).
AI-pushed automatic picture Evaluation Along with the Attune Cytometric Program translates occasion attributes into distinctive morphology parameters which can be combined with common fluorescence and scatter parameters to expose remarkably reproducible inhabitants improvements and distinctive subsets.
We previously demonstrated the power of imaging Car or truck-T/Ramos cell interactions. Permit’s have a look at just the population of finest interest, the double beneficial functions, To find out more. We could now use extended morphological parameters (in this article, circularity and skewness of depth) to further more look at the options of those populations and refine gating on these gatherings, increasing facts robustness.
Constructed-in dimensionality reduction applications increased resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to verify anticipated inhabitants resolution in lysed complete human blood. Consumer can watch activities from Each and every cluster for Visible affirmation of cell phenotypes.
Flexible payment strategy from Agilent Money Solutions enable you to have read more the significant instrumentation you will need for speedy venture, or to grow your lab.
Gating on these prolonged parameters permits you to immediately and accurately recognize populations of desire and make sure gating tactic with little if any guide assessment. The Attune program delivers usage of point out-of-the-art device Discovering analysis applications within an expertly made consumer interface, making it extremely convenient to use.
The Attune Xenith cytometer is often a higher-parameter spectral analyzer, providing each spectral unmixing and standard flow capabilities for in depth and flexible cell Examination.
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Right here, we display that by gating approaches utilizing impression-based quantitative parameters can distinguish interacting cells from coincident activities to a lot more correctly review interacting cells.